Lung epithelial GM-CSF improves host defense function and epithelial repair in influenza virus pneumonia—a new therapeutic strategy?
© The Author(s). 2016
Received: 2 May 2016
Accepted: 15 July 2016
Published: 1 August 2016
Influenza viruses (IVs) circulate seasonally and are a common cause of respiratory infections in pediatric and adult patients. Additionally, recurrent pandemics cause massive morbidity and mortality worldwide. Infection may result in rapid progressive viral pneumonia with fatal outcome. Since accurate treatment strategies are still missing, research refocuses attention to lung pathology and cellular crosstalk to develop new therapeutic options.
Alveolar epithelial cells (AECs) play an important role in orchestrating the pulmonary antiviral host response. After IV infection they release a cascade of immune mediators, one of which is granulocyte and macrophage colony-stimulating factor (GM-CSF). GM-CSF is known to promote differentiation, activation and mobilization of myeloid cells. In the lung, GM-CSF drives immune functions of alveolar macrophages and dendritic cells (DCs) and also improves epithelial repair processes through direct interaction with AECs. During IV infection, AEC-derived GM-CSF shows a lung-protective effect that is also present after local GM-CSF application. This mini-review provides an overview on GM-CSF-modulated immune responses to IV pneumonia and its therapeutic potential in severe IV pneumonia.
Respiratory viral infections are often seen in pediatric patients . They may cause primary viral pneumonia that may progress to lung failure with fatal outcome. Apart from respiratory syncytial virus (RSV) and other respiratory viruses, influenza virus (IV) infection is a common cause of acute respiratory failure on pediatric intensive care units (ICUs) .
IVs are enveloped single-stranded negative-sensed RNA viruses, divided into three genera A, B, and C . They cause respiratory infections in humans and not only occur seasonally but also occur in recurrent pandemics . IV A is further divided into subtypes that differ in the surface glycoproteins hemagglutinin (HA) and neuraminidase (NA). The viral HA serves to attach to host cells; the NA protein cares for viral release after replication . Due to the segmented nature of its genome and a high rate of mutations during replication, seasonal IVs show an annual change of antigenic qualities in the HA and NA genes, requiring annual immunization. Mutations in the coding genes for HA and NA are called antigenic drift, while the reassortment of viral gene segments is called antigenic shift . The creation of new viral subtypes by reassortment is only known to be prevalent in type A IVs with a large reservoir of different strains in animals, particularly waterfowl but also in other birds and mammals .
Primary target cells for IV infection in humans are respiratory epithelial cells . The current opinion is that the major route of infection proceeds by viral HA binding to sialic acid receptors on the epithelial cells, followed by internalization via endocytosis . The viral genome is replicated in the nucleus and translated in the cytoplasm, and mature virus particles are released from the cell via budding .
As soon as the infection spreads from the upper to the lower respiratory tract, alveolar epithelial cells (AECs) become primary targets for productive IV replication . Pro-inflammatory mechanisms—together with a direct viral cytopathogenic effect—lead to AEC apoptosis. Additionally, lung pathology is characterized by loss of alveolar barrier function and alveolar edema fluid accumulation . Ongoing inflammation leads to increased capillary/alveolar leakage, followed by severe hypoxemia  and results in acute respiratory distress syndrome in children (PARDS) and adults .
Rapid and effective viral clearance from the distal lung by immune effector cells and the initiation of epithelial repair processes including expansion of local epithelial progenitor cells and resealing of the epithelial layer are crucial to recover from IV-induced lung injury [11, 12]. The inflammatory immune response needs to be balanced between the elimination of virus and immune-mediated pulmonary injury to limit the damage to the respiratory tract .
Myeloid cells like monocytes, macrophages, dendritic cells (DCs), and their common precursor cells, summarized as mononuclear phagocytes, are crucial in driving IV clearance . Combining sensor and effector functions of innate immunity, the lung epithelium plays an important role in coordinating, maintaining, and balancing the phagocyte-mediated antiviral host response [7, 14, 15]. The intimate spatial proximity of alveolar macrophages and the tissue-resident DC network with the distal lung epithelium provides an ideal basis for direct cell-cell communication. Mechanisms involved in this cellular crosstalk might represent potential targets for treatment.
Pulmonary GM-CSF and its secretion during IV infection
The growth factor granulocyte and macrophage colony-stimulating factor (GM-CSF) is widely recognized to promote proliferation, differentiation, and activation of monocytes, granulocytes, macrophages, and DCs in vivo and plays an important role in immunity [16, 17]. Involved in proinflammatory cytokine response, it is discussed to be responsible for immunopathology in several inflammatory or autoimmune diseases . It controls nonlymphoid tissue DC homeostasis  and was currently reported to modulate the development of inflammatory macrophages and monocytes . In the lung, GM-CSF was shown not only to play a role in allergic airway disease [20, 21] but also to be crucial for antimicrobial pulmonary host defense function [22–24]. Additionally, it is essential for surfactant homeostasis. GM-CSF-deficient mice develop a similar pathology to human pulmonary alveolar proteinosis (PAP) . An exceeded accumulation of surfactant phospholipids and proteins in the alveolar lining fluid impairs gas exchange and patients show increased susceptibility for microbial infections . PAP is found to be associated with GM-CSF or GM-CSF receptor auto-antibodies or dysfunction .
Pulmonary GM-CSF is mainly expressed by AEC type II and released under inflammatory conditions . Other cell types like macrophages, endothelial cells, fibroblasts and T cells also produce GM-CSF, but AECs were shown to be the only CD45− cell population in the distal lung parenchyma that upregulates GM-CSF upon IV infection, and produces high levels of GM-CSF in the alveolar lining fluid . New findings report AEC GM-CSF secretion in IV infection to be mediated through HGF/c-Met and TGF-α/epithelial growth factor receptor (EGFR) signaling .
AEC-derived GM-CSF is highly protective in IV pneumonia
It is well established that AEC-released GM-CSF improves innate immune responses of myeloid cells, in particular alveolar macrophages. Alveolar macrophages of GM-CSF-deficient mice show abnormalities in morphology, maturation, and function, depending on transcription factor PU.1 [22, 26]. Sever-Chroneos et al.  found a decreased resistance to IV in GM-CSF-deficient mice due to impaired pathogen clearance by macrophages. This is stressed by findings from Berclaz et al. who demonstrated that the Fcɣ receptor (FcɣR)-mediated opsonophagocytosis of invaded pathogens by alveolar macrophages is highly dependent on GM-CSF signaling via PU.1 . T cell-produced interferon ɣ (IFN ɣ) during course of infection also effected augmented FcɣR levels on alveolar macrophages which in turn stimulated IFN ɣ production by secretion of inflammatory cytokines (interleukin (IL)-18, IL-12), linking innate and adaptive immunity in a positive feedback loop. Elevated alveolar GM-CSF level in transgenic mice increased numbers and resistance of alveolar macrophages and provided protection against lethal IV infections . Subramaniam et al.  observed a GM-CSF-dependent protection through stimulation of reactive oxygen species (ROS) production by macrophages not only against IV pneumonia itself but also against secondary bacterial pneumonia, a major cause of morbidity and mortality after IV infection. However, GM-CSF-dependent activation of pulmonary innate immunity does not explain the beneficial effects of AEC GM-CSF in IV infection in total. Lung CD103+ DCs were found to be key players for the GM-CSF-dependent lung protective effect by our group . After IV infection, pulmonary CD103+ DCs are expanded and activated and their migration and antigen (Ag) presentation to the draining mediastinal lymph nodes is mediated by GM-CSF. This is associated with a better viral clearance and Ag-specific T cell recruitment, suggesting improved resident lung DC host defense capacities during IV infection by AEC GM-CSF. Min et al.  identified GM-CSF to be a major licensing factor of CD8+ T lymphocytes to activate DCs during priming in lymphoid tissue, providing a positive feedback loop in the stimulation of CD8+ T cell proliferation. Accordingly, Greter et al.  revealed GM-CSF to be indispensable for the induction of specific CD8+ T cell immunity. Chen et al.  also found GM-CSF to promote T cell, B cell, and DC maturation in order to enable the production of IV specific antibodies.
During IV pneumonia, GM-CSF furthermore has a lung barrier-protective effect and improves survival, also after local application [28, 30, 32, 36]. AEC-expressed GM-CSF has direct beneficial effects on the injured epithelium. It is crucial in mediating epithelial proliferation in inflammatory or hyperoxic lung injury, supports repair and restoration of barrier function, and induces the return to tissue homeostasis [27, 37]. In a bleomycin rat model, it was shown that defects in AEC II GM-CSF secretion are crucial for pathogenesis of pulmonary fibrosis . Sturrock et al. displayed certain microRNAs (miRNAs) (133a and 133b) to suppress protective AEC GM-CSF secretion by inhibition of GM-CSF messenger RNA (mRNA) translation in oxidative stress . This effect seemed to be AEC specific as the same miRNAs did not suppress T cell GM-CSF expression during hyperoxia.
In adult patients with acute respiratory distress syndrome (ARDS), elevated GM-CSF levels in the bronchoalveolar lavage fluid (BALF) were shown to be associated with antiapoptotic effects and with improved epithelial barrier integrity and survival [40, 41]. In human lung sections, GM-CSF expressed by hyperplastic type II AECs mediates accumulation of neighboring CD1a+ DCs in inflamed lungs . These findings suggest that GM-CSF is similarly beneficial and operative in humans. Of note, high serum cytokine levels of GM-CSF in pediatric intensive care unit (ICU) patients with severe influenza infection were associated with innate immune suppression and mortality , highlighting the particular beneficial effect of alveolar as opposed to circulating GM-CSF.
GM-CSF as a therapeutic strategy
Current treatment strategies for IV infection focus on vaccines and antiviral agents. Due to its rapid genetic modification capacity, it will be a challenge to keep up with therapeutic targets as resistance rapidly occurs against current antivirals. Immune mediators that drive pulmonary host defense function like GM-CSF propose appealing alternative treatment strategies, especially if they do not only protect against IV infection itself but also against common complications like secondary bacterial pneumonia and severe damage of alveolar epithelium.
A clinical trial for low-dose intravenous GM-CSF treatment in adult patients with severe sepsis and respiratory dysfunction led to the conclusion that GM-CSF treatment was associated with improved gas exchange and might play a homeostatic role . Another trial in adult patients with acute lung injury (ALI) failed to improve survival and ventilation parameters by intravenous GM-CSF application . Regarding the mouse model, a high local concentration of GM-CSF in the alveolar lining fluid seems to be more promising than systemic application and also prevents systemic side effects. Alveolar GM-CSF is required in a distinct local quantity to balance pathogen clearance and immunopathology. This is why GM-CSF treatment should preferentially be investigated via the inhaled route in contrast to systemic application. Inflammation itself leads to compromised lung barrier integrity, causing systemic loss of locally delivered GM-CSF. Current strategies in the mouse model focus on GM-CSF conjugation to enhance bioavailability, prolong half-life, and reduce systemic side-effects of administered GM-CSF .
For patients with PAP, inhaled GM-CSF therapy displayed encouraging results [47, 48]. With regard to pneumonia-associated ARDS an off-label treatment with inhaled GM-CSF revealed an improvement in oxygenation and morbidity . GM-CSF treatment increased activation of alveolar macrophages, important for host defense function. A multicenter, double-blind, placebo-controlled, randomized clinical trial on the efficacy of inhaled GM-CSF in adult patients with pneumonia-associated ARDS (GI-HOPE) was recently started within the nationwide German Center for Lung Research (NCT02595060). Similarly, an interventional safety/efficacy study for inhaled GM-CSF treatment in children with respiratory virus-associated ARDS has been implemented (iGRASP, NCT02601365), highlighting the strong potential of this drug in both adults and children suffering from severe viral pneumonia and related ARDS.
This review focuses on GM-CSF-mediated effects in IV pneumonia and associated ARDS. The clinical trials mentioned above investigate GM-CSF treatment not exclusively in IV pneumonia but in (viral) pneumonia-associated ARDS in general. While in the mouse model, specific conditions can be studied in detail, clinical conditions are different. In the clinical setting, the underlying germ of infection is not always clearly defined when patients present with pneumonia/ARDS and therapy needs to be initiated. Furthermore, bacterial superinfection of primary viral pneumonia is a common complication. GM-CSF-mediated protective effects are not limited to IV infection as they result in improvement of host defense capacities and epithelial repair in general, both critical processes in ARDS patients. GM-CSF might be working similarly in other respiratory viral infections as well. It was reported to prevent RSV-exacerbated airway hyperresponsiveness by alveolar macrophage maturation , for example. Protective effects of GM-CSF were also shown for bacterial pneumonia [23, 51, 52]. For these reasons, clinical studies focus on GM-CSF treatment on patients, who present with pneumonia-associated ARDS rather than exclusively IV pneumonia.
Local therapeutic application of GM-CSF increases mononuclear phagocyte-mediated innate and adaptive host defense and accelerates epithelial repair processes during severe IV pneumonia in pre-clinical models. There is evidence that it might be a powerful therapy in viral (and bacterial) pneumonia and associated ARDS and eventually even other forms of ARDS in children and adults. Current data suggest that local application by the inhalative route seems to be most promising.
AEC, alveolar epithelial cell; Ag, antigen; ALI, acute lung injury; AM, alveolar macrophage; ARDS, acute respiratory distress syndrome; BALF, bronchoalveolar lavage fluid; c-Met, hepatocyte growth factor receptor; DC, dendritic cell; EGFR, epithelial growth factor receptor; FcɣR, Fcɣ receptor; GI-HOPE, clinical trial on GM-CSF treatment in ARDS patients; GM-CSF, granulocyte and macrophage colony stimulating factor; HA, hemagglutinin; HGF, hepatocyte growth factor; ICU, intensive care unit; IFNγ, interferon-γ; iGRASP, clinical trial on GM-CSF treatment in children with virus-associated ARDS; IL, interleukin; IV, influenza virus; miRNA, microRNA; mRNA, messenger RNA; NA, neuraminidase; PAP, pulmonary alveolar proteinosis; PARDS, pediatric acute respiratory distress syndrome; PU.1, transcription factor PU.1; ROS, reactive oxygen species; RSV, respiratory syncytial virus; TGF α, transcriptional growth factor α
This work was supported by the German Center for Lung Research (DZL), the Excellence Cluster Cardio-Pulmonary System (EXC147), and the Collaborative Research Centers SFB1021 and SFBTR-84 (DFG).
The authors disclose any financial or non-financial competing interests. They have nothing to declare.
Both authors read and approved the final manuscript.
Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made.
- Heikkinen T (2016) Respiratory viruses and children. J Infect 72(Suppl):S29–S33View ArticlePubMedGoogle Scholar
- Nair H, Brooks WA, Katz M, Roca A, Berkley JA, Madhi SA, Simmerman JM, Gordon A, Sato M, Howie S, Krishnan A, Ope M, Lindblade KA, Carosone-Link P, Lucera M, Ochieng W, Kamimota L, Dueger E, Bhat N, Vong S, Theodoratou E, Chittaganpitch M, Chimah O, Balmaseda A, Buchy P, Harris E, Evans V, Katayose M, Gaur B, O’Callaghan-Gordo C, Goswami D, Arvelo W, Venter M, Briese T, Tokarz R, Widdowson MA, Mounts AW, Breiman RF, Feikin DR, Klugman KP, Olsen SJ, Gessner BD, Wright PF, Rudan I, Broor S, Simoes EAF, Campbell H (2011) Global burden of respiratory infections due to seasonal influenza in young children: a systematic review and meta-analysis. Lancet 378(9807):1917–1930View ArticlePubMedGoogle Scholar
- Bouvier NM, Palese P (2008) The biology of influenza viruses. Vaccine 26(Suppl 4):D49–D53View ArticlePubMedPubMed CentralGoogle Scholar
- Kuiken T, Taubenberger JK (2008) Pathology of human influenza revisited. Vaccine 26(suppl 4):D59–D66View ArticlePubMedPubMed CentralGoogle Scholar
- Medina RA, García-Sastre A (2011) Influenza A viruses: new research developments. Nat Rav Microbiol 9(8):590–603View ArticleGoogle Scholar
- Klenk HD (2014) Influenza viruses en route from birds to man. Cell Host Microbe 15(6):653–654View ArticlePubMedGoogle Scholar
- Sanders CJ, Doherty PC, Thomas PG (2010) Respiratory epithelial cells in innate immunity to influenza virus infection. Cell Tissue Res 343(1):13–21View ArticlePubMedGoogle Scholar
- Dash P, Thomas PG (2015) Host detection and the stealthy phenotype in influenza virus infection. Curr Top Microbiol Immunol 386:121–147PubMedGoogle Scholar
- Mauad T, Hajjar LA, Callegari GD, da Silva LFF, Schout D, Galas FRBG, Alves VAF, Malheiros DMAC, Auler JOC Jr, Ferreira AF, Borsato MRL, Bezerra SM, Gutierrez PS, Caldini ETEG, Pasqualucci CA, Dolhnikoff M, Saldiva PHN (2010) Lung pathology in fatal novel human influenza A (H1N1) infection. Am J Respir Crit Care Med 181(1):72–79View ArticlePubMedGoogle Scholar
- Streng A, Prifert C, Weissbrich B, Liese JG, Bavarian PICU Study Group on Influenza and Other Viral ARI (2015) Continued high incidence of children with severe influenza A(H1N1)pdm09 admitted to paediatric intensive care units in Germany during the first three post-pandemic influenza seasons, 2010/11–2012/13. BMC Infect Dis 15:573View ArticlePubMedPubMed CentralGoogle Scholar
- Gorski SA, Hufford MM, Braciale TJ (2012) Recent insights into pulmonary repair following virus-induced inflammation of the respiratory tract. Curr Opin Virol 2(3):233–241View ArticlePubMedPubMed CentralGoogle Scholar
- Braciale TJ, Sun J, Kim TS (2012) Regulating the adaptive immune response to respiratory virus infection. Nat Rev Immunol 12(4):295–305View ArticlePubMedPubMed CentralGoogle Scholar
- Newton AH, Cardani A, Braciale TJ (2016) The host immune response in respiratory virus infection: balancing virus clearance and immunopathology. Semin Immunopathol 38:471–482View ArticlePubMedPubMed CentralGoogle Scholar
- Hussell T, Goulding J (2010) Structured regulation of inflammation during respiratory viral infection. Lancet Infect Dis 10(5):360–366View ArticlePubMedGoogle Scholar
- La Gruta NL, Kedzierska K, Stambas J, Doherty PC (2007) A question of self-preservation: immunopathology in influenza virus infection. Immunol Cell Biol 85(2):85–92View ArticlePubMedGoogle Scholar
- Hamilton JA (2008) Colony-stimulating factors in inflammation and autoimmunity. Nat Rev Immunol 8(7):533–544View ArticlePubMedGoogle Scholar
- Hamilton JA, Achuthan A (2013) Colony stimulating factors and myeloid cell biology in health and disease. Trends Immunol 34(2):81–89View ArticlePubMedGoogle Scholar
- Greter M, Helft J, Chow A, Hashimoto D, Mortha A, Agudo-Cantero J, Bogunovic M, Gautier EL, Miller J, Leboeuf M, Lu G, Aloman C, Brown BD, Pollard JW, Xiong H, Randolph GJ, Chipuk JE, Frenette PS, Merad M (2012) GM-CSF controls nonlymphoid tissue dendritic cell homeostasis but is dispensable for the differentiation of inflammatory dendritic cells. Immunity 36(6):1031–1046View ArticlePubMedPubMed CentralGoogle Scholar
- Louis C, Cook AD, Lacey D, Fleetwood AJ, Vlahos R, Anderson GP, Hamilton JA (2015) Specific contributions of CSF-1 and GM-CSF to the dynamics of the mononuclear phagocyte system. J Immunol 195(1):134–144View ArticlePubMedGoogle Scholar
- Cates EC, Fattouh R, Wattie J, Inman MD, Goncharova S, Coyle AJ, Gutierrez-Ramos JC, Jordana M (2004) Intranasal exposure of mice to house dust mite elicits allergic airway inflammation via a GM-CSF-mediated mechanism. J Immunol 173:6384–6392View ArticlePubMedGoogle Scholar
- Willart MAM, Deswarte K, Pouliot P, Braun H, Beyaert R, Lambrecht BN, Hammad H (2012) Interleukin-1α controls allergic sensitization to inhaled house dust mite via the epithelial release of GM-CSF and IL-33. J Exp Med 209(8):1505–1517View ArticlePubMedPubMed CentralGoogle Scholar
- Trapnell BC, Whitsett JA (2002) GM-CSF regulates pulmonary surfactant homeostasis and alveolar macrophage-mediated innate host defense. Annu Rev Physiol 64:775–802View ArticlePubMedGoogle Scholar
- Ballinger MN, Paine R III, Serezani CHC, Aronoff DM, Choi ES, Standiford TJ, Toews GB, Moore BB (2006) Role of granulocyte macrophage colony-stimulating factor during gram-negative lung infection with Pseudomonas aeruginosa. Am J Respir Cell Mol Biol 34(6):766–774View ArticlePubMedPubMed CentralGoogle Scholar
- Chen GH, Teitz-Tennenbaum S, Neal LM, Murdock BJ, Malachowski AN, Dils AJ, Olszewski MA, Osterholzer JJ (2016) Local GM-CSF-dependent differentiation and activation of pulmonary dendritic cells and macrophages protect against progressive cryptococcal lung infection in mice. J Immunol 196:1810–1821View ArticlePubMedGoogle Scholar
- Stanley E, Lieschke GJ, Grail D, Metcalf D, Hodgson G, Galle JAM, Maher DW, Cebon J, Sinickas V, Dunn AR (1994) Granulocyte/macrophage colony-stimulating factor-deficient mice show no major perturbation of hematopoiesis but develop a characteristic pulmonary pathology. Proc Natl Acad Sci 91:5592–5596View ArticlePubMedPubMed CentralGoogle Scholar
- Trapnell BC, Carey BC, Uschida K, Suzuki T (2009) Pulmonary alveolar proteinosis, a primary immunodeficiency of impaired GM-CSF stimulation of macrophages. Curr Opin Immunol 21(5):514–521View ArticlePubMedPubMed CentralGoogle Scholar
- Cakarova L, Cakarova L, Marsh LM, Wilhelm J, Mayer K, Grimminger F, Seeger W, Lohmeyer J, Herold S (2009) Macrophage tumor necrosis factor-alpha induces epithelial expression of granulocyte-macrophage colony-stimulating factor: impact on alveolar epithelial repair. Am J Respir Crit Care Med 180(6):521–532View ArticlePubMedGoogle Scholar
- Unkel B, Hoegner K, Clausen BE, Lewe-Schlosser P, Bodner J, Gattenloehner S, Janßen H, Seeger W, Lohmeyer J, Herold S (2012) Alveolar epithelial cells orchestrate DC function in murine viral pneumonia. J Clin Invest 122(10):3652–3664View ArticlePubMedPubMed CentralGoogle Scholar
- Ito Y, Correll K, Zemans RI, Leslie CC, Murphy RC, Mason RJ (2015) Influenza induces IL-8 and GM-CSF secretion by human alveolar epithelial cells through HGF/c-Met and TGF-a/EGFR signaling. Am J Physiol Lung Cell Mol Physiol 308(11):L1178–L1188View ArticlePubMedPubMed CentralGoogle Scholar
- Sever-Chroneos Z, Murthy A, Davis J, Florence JM, Kurdowska A, Krupa A, Tichelaar JW, White MR, Hartshorn KL, Kobzik L, Whitsett JA, Chroneos ZC (2011) GM-CSF modulates pulmonary resistance to influenza A infection. Antiviral Res 92(2):319–328View ArticlePubMedPubMed CentralGoogle Scholar
- Berclaz PY, Shibata Y, Whitsett JA, Trapnell BC (2002) GM-CSF, via PU.1, regulates alveolar macrophage FcγR-mediated phagocytosis and the IL-18/IFN-γ-mediated molecular connection between innate and adaptive immunity in the lung. Blood 100(12):4193–4200View ArticlePubMedGoogle Scholar
- Huang FF, Barnes PF, Feng Y, Donis R, Chroneos ZC, Idell S, Allen T, Perez DR, Whitsett JA, Dunussi-Joannopoulos K, Shams H (2011) GM-CSF in the lung protects against lethal influenza infection. Am J Respir Crit Care Med 184(2):259–268View ArticlePubMedGoogle Scholar
- Subramaniam R, Barnes PF, Fletcher K, Boggaram V, Hillberry Z, Neuenschwander P, Shams H (2014) Protecting against post-influenza bacterial pneumonia by increasing phagocyte recruitment and ROS production. J Infect Dis 209:1827–1836View ArticlePubMedGoogle Scholar
- Min L, Mohammad Isa SAB, Shuai W, Piang CB, Nih FW, Kotaka M, Ruedl C (2011) Cutting edge: granulocyte-macrophage colony-stimulating factor is the major CD8+ T cell derived licensing factor for dendritic cell activation. J Immunol 184(9):4625–4629View ArticleGoogle Scholar
- Chen Q, He F, Kwang J, Chan JKY, Chen J (2012) GM-CSF and IL-4 stimulate antibody responses in humanized mice by promoting T, B, and dendritic cell maturation. J Immunol 189:5223–5229View ArticlePubMedPubMed CentralGoogle Scholar
- Huang H, Li H, Zhou P, Ju D (2010) Protective effects of recombinant human granulocyte macrophage colony stimulating factor on H1N1 influenza virus-induced pneumonia in mice. Cytokine 51(2):151–157View ArticlePubMedGoogle Scholar
- Paine R III, Wilcoxen SE, Morris SB, Sartori C, Baleeiro CEO, Matthay MA, Christensen PJ (2003) Transgenic overexpression of granulocyte macrophage-colony stimulating factor in the lung prevents hyperoxic lung injury. Am J Pathol 163(6):2397–2406View ArticlePubMedPubMed CentralGoogle Scholar
- Christensen PJ, Bailie MB, Goodman RE, O’Brien AD, Toews GB, Paine R III (2000) Role of diminished epithelial GM-CSF in the pathogenesis of bleomycin-induced pulmonary fibrosis. Am J Physiol Lung Cell Mol Physiol 279:L487–L495PubMedGoogle Scholar
- Sturrock A, Mir-Kasimov M, Baker J, Rowley J, Paine R III (2014) Key role of microRNA in the regulation of granulocyte macrophage colony-stimulating factor expression in murine alveolar epithelial cells during oxidative stress. J Biol Chem 289(7):4095–4105View ArticlePubMedGoogle Scholar
- Matute-Bello G, Liles WC, Radella F 2nd, Steinberg KP, Ruzinski JT, Hudson LD, Martin TR (2000) Modulation of neutrophil apoptosis by granulocyte colony-stimulating factor and granulocyte/macrophage colony-stimulating factor during the course of acute respiratory distress syndrome. Crit Care Med 28(1):1–7View ArticlePubMedGoogle Scholar
- Overgaard CE, Schlingmann B, Dorsainvil White SC, Ward C, Fan X, Swarnakar S, Brown LA, Guidot DM, Koval M (2015) The relative balance of GM-CSF and TGF-β1 regulates lung epithelial barrier function. Am J Physiol Lung Cell Mol Physiol 308(12):L1212–L1223View ArticlePubMedPubMed CentralGoogle Scholar
- Tazi A, Bouchonnet F, Grandsaigne M, Boumsell L, Hance AJ, Soler P (1993) Evidence that granulocyte macrophage-colony-stimulating factor regulates the distribution and differentiated state of dendritic cells/Langerhans cells in human lung and lung cancers. J Clin Invest 91(2):566–576View ArticlePubMedPubMed CentralGoogle Scholar
- Hall MW, Geyer SM, Guo CY, Panoskaltsis-Mortari A, Jouvet P, Ferdinands J, Shay DK, Nateri J, Greathouse K, Sullivan R, Tran T, Keisling S, Randolph AG, Pediatric Acute Lung Injury and Sepsis Investigators (PALISI) Network PICFlu Study Investigators (2013) Innate immune function and mortality in critically ill children with influenza: a multicenter study. Crit Care Med 41(1):224–236View ArticleGoogle Scholar
- Presneill JJ, Harris T, Stewart AG, Cade JF, Wilson JW (2002) A randomized phase II trial of granulocyte-macrophage colony-stimulating factor therapy in severe sepsis with respiratory dysfunction. Am J Respir Crit Care Med 166:138–143View ArticlePubMedGoogle Scholar
- Paine R III, Standiford TJ, Dechert RE, Moss M, Martin GS, Rosenberg AL, Thannickal VJ, Burnham EL, Brown MB, Hyzy RC (2012) A randomized trial of recombinant human GM-CSF for patients with acute lung injury. Crit Care Med 40(1):90–97View ArticlePubMedPubMed CentralGoogle Scholar
- Subramaniam R, Hillberry Z, Chen H, Feng Y, Fletcher K, Neuenschwander P, Shamns H (2015) Delivery of GM-CSF to protect against influenza pneumonia. PLoS One 10(4):e0124593View ArticlePubMedPubMed CentralGoogle Scholar
- Tazawa R, Trapnell BC, Inoue Y, Arai T, Takada T, Nasuhara Y, Hizawa N, Kasahara Y, Tatsumi K, Hojo M, Ishii H, Yokoba M, Tanaka N, Yamaguchi E, Eda R, Tsuchihashi Y, Morimoto K, Akira M, Terada M, Otsuka J, Ebina M, Kaneko C, Nukiwa T, Krischer JP, Akazawa K, Nakata K (2010) Inhaled granulocyte/macrophage-colony stimulating factor as therapy for pulmonary alveolar proteinosis. Am J Respir Crit Care Med 181:1345–1354View ArticlePubMedPubMed CentralGoogle Scholar
- Khan A, Agarwal R, Aggarwal AN (2012) Effectiveness of granulocyte-macrophage colony-stimulating factor therapy in autoimmune pulmonary alveolar proteinosis. Chest 141(5):1273–1283View ArticlePubMedGoogle Scholar
- Herold S, Hoegner K, Vadász I, Gessler T, Wilhelm J, Mayer K, Morty RE, Walmrath HD, Seger W, Lohmeyer J (2014) Inhaled granulocyte/macrophage colony-stimulating factor as treatment of pneumonia-associated acute respiratory distress syndrome. Am J Respir Crit Care Med 189(1):609–611View ArticlePubMedGoogle Scholar
- Naessens T, Schepens B, Smet M, Pollard C, Van Hoecke L, De Beuckelaer A, Willart M, Lambrecht B, De Koker S, Saelens X, Grooten J (2016) GM-CSF treatment prevents respiratory syncytial virus-induced pulmonary exacerbation responses in postallergic mice by stimulating alveolar macrophage maturation. J Allergy Clin Immunol 137(3):700–709View ArticlePubMedGoogle Scholar
- Steinwede K, Tempelhof O, Bolte K, Maus R, Bohling J, Ueberberg B, Länger F, Christman JW, Paton JC, Ask K, Maharaj S, Kolb M, Gauldie J, Welte T, Maus UA (2011) Local delivery of granulocyte/macrophage colony stimulating factor protects mice from lethal pneumococcal pneumonia. J Immunol 187(10):5346–5356View ArticlePubMedPubMed CentralGoogle Scholar
- Standiford LR, Standiford TJ, Newstead MJ, Zeng X, Ballinger MN, Kovach MA, Reka AK, Bhan U (2012) TLR4-dependent GM-CSF protects against lung injury in Gram-negative bacterial pneumonia. Am J Physiol Lung Cell Mol Physiol 302:L447–L454View ArticlePubMedGoogle Scholar