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Table 3 Biological sampling methods and analyses

From: KUNO-Kids birth cohort study: rationale, design, and cohort description

Specimen Purpose Downstream applications
Cord blood—EDTA Genomic DNA isolation Genotyping, sequencing, epigenetics (methylation) analyses
Cord blood—PAXgene RNA and miRNA isolation Transcriptomics
Cord blood—SSR (Clot Activator Tube) Serum separation Protein measurements: cytokines, allergens; metabolomics (NMR, LC-MS)
Cord blood—Li-Heparin Plasma separation Protein measurements: cytokines, allergens; metabolomics (NMR, LC-MS)
Stool Bacterial DNA isolation Microbiome analyses
Urine   Metabolomics (NMR, LC-MS)
Skin swabs—cheek, elbow, forearm Bacterial DNA isolation Microbiome analyses
Buccal swabs Genomic DNA isolation Genotyping, sequencing, epigenetics (methylation) analyses
Buccal swabs RNA and miRNA isolation Transcriptomics
Hair   Toxicology analyses
Gingival smears Bacterial DNA isolation Microbiome analyses
  1. Note: All biosamples are collected from the child at baseline