Table 3 Biological sampling methods and analyses
From: KUNO-Kids birth cohort study: rationale, design, and cohort description
Specimen | Purpose | Downstream applications |
|---|---|---|
Cord blood—EDTA | Genomic DNA isolation | Genotyping, sequencing, epigenetics (methylation) analyses |
Cord blood—PAXgene | RNA and miRNA isolation | Transcriptomics |
Cord blood—SSR (Clot Activator Tube) | Serum separation | Protein measurements: cytokines, allergens; metabolomics (NMR, LC-MS) |
Cord blood—Li-Heparin | Plasma separation | Protein measurements: cytokines, allergens; metabolomics (NMR, LC-MS) |
Stool | Bacterial DNA isolation | Microbiome analyses |
Urine | Metabolomics (NMR, LC-MS) | |
Skin swabs—cheek, elbow, forearm | Bacterial DNA isolation | Microbiome analyses |
Buccal swabs | Genomic DNA isolation | Genotyping, sequencing, epigenetics (methylation) analyses |
Buccal swabs | RNA and miRNA isolation | Transcriptomics |
Hair | Toxicology analyses | |
Gingival smears | Bacterial DNA isolation | Microbiome analyses |