Volume 2 Supplement 1

Abstracts of the 51st Workshop for Pediatric Research

Open Access

Differential expression of mucosal trefoil factors and mucins in pediatric inflammatory bowel diseases

  • Andreas C Jenke1,
  • Veronika Boland1,
  • Jan Postberg1,
  • Silvia Vogel2,
  • Daniel Gödde2,
  • Matthias Zilbauer3,
  • Robert Heuschkel3,
  • Stefan Wirth1 and
  • Kai O Hensel1
Molecular and Cellular Pediatrics20152(Suppl 1):A5

https://doi.org/10.1186/2194-7791-2-S1-A5

Published: 1 July 2015

Aims

In the intestinal mucosa trefoil factors (TFF) and mucins (Muc) - primarily produced by goblet cells - are thought to play a major role in providing barrier function during infection and inflammation. To investigate their role in pediatric Crohn's disease (CD) and ulcerative colitis (UC).

Methods

We obtained mucosal biopsies of children with CD, UC and healthy controls, analyzed genetic expression using real-time PCR and related the outcomes to clinical data. Subsequently, immunohistochemistry was utilized to verify protein expression in biopsy specimens.

Results

Levels of TFF2 mRNA were lower in inflamed mucosal samples (terminal ileum (TI) and duodenum) of children with CD, but higher in non-inflamed mucosal samples when compared to healthy controls (p < 0.05). Similarly, TFF2 levels in the TI were significantly lower in inflamed UC tissue. Adjustment for goblet cell density revealed slightly less marked, yet significantly different gene expression in IBD and controls. Furthermore, TI expression of TFF2 and Muc2 was inversely correlated with interleukin-8 expression in CD (p = 0.027).

Conclusion

Our data demonstrate significant changes in Muc and TFF mRNA expression in pediatric patients with IBD suggesting a role in mucosal healing. Further studies are needed to elucidate a potential use as biomarkers for disease progression.

Authors’ Affiliations

(1)
Department of Pediatrics, HELIOS Medical Center Wuppertal, Faculty of Health, Witten/Herdecke University
(2)
Department of Pathology, HELIOS Medical Center Wuppertal, Faculty of Health, Witten/Herdecke University
(3)
Department of Pediatric Gastroenterology, Hepatology and Nutrition, Addenbrooke's Hospital, University Department of Pediatrics, University of Cambridge

Copyright

© Jenke et al. 2015

This article is published under license to BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.