Molecular detection and characterization of Shigella spp. harboring extended-spectrum β-lactamase genes in children with diarrhea in northwest Iran

Molecular and Cellular Pediatrics

Table 1 Primers used for the detection of the Shigella species and ESBL encoding genes

Primers name		Sequence (5′→3′)	PCR product size (bp)	References
invC	F	TGCCCAGTTTCTTCATACGC	879	[17]
invC	R	GAAAGTAGCTCCCGAAATGC	879	[17]
wbgZ	F	TCTGAATATGCCCTCTAC	430	[17]
wbgZ	R	GACAGAGCCCGAAGAACCG	430	[17]
rfpB	F	TCTCAATAATAGGGAACACAGC	537	[17]
rfpB	R	CATAAATCACCAGCAAGGTT	537	[17]
rfc	F	TTTATGGCTTCTTTGTCG	211	[17]
rfc	R	CTGCGTGATCCGACCATG	211	[17]
ipaH	F	GTTCCTTGACCGCCTTTCCGATACCGTC	619	[18]
ipaH	R	GCCGGTCAGCCACCCTCTGAGAGTAC	619	[18]
bla_SHV	F	ATTTGTCGCTTCTTTACTCGC	1018	[19]
bla_SHV	R	TTTATGGCGTTACCTTTGACC	1018	[19]
bla_CTX-M	F	ATGTGCAGYACCAGTAARGT	544	[19]
bla_CTX-M	R	TGGGTRAARTARGTSACCAGA	544	[19]
bla_TEM	F	ATAAAATTCTTGAAGACGAAA	1076	[19]
bla_TEM	R	GACAGTTACCAATGCTTAATC	1076	[19]
ERIC	F	ATGTAAGCTCCTGGGGATTCAC	Variable	[20]
ERIC	R	AAGTAAGTGACTGGGGTGAGCG	Variable	[20]