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Molecular and Cellular Pediatrics
Fig. 3 | Molecular and Cellular Pediatrics

Fig. 3

From: Combined RT-qPCR and pyrosequencing of a Spike glycoprotein polybasic cleavage motif can uncover pediatric SARS-CoV-2 infections associated with heterogeneous presentation

Fig. 3

Comparative characterization of RT-qPCR efficiency and sensitivity. The same clinical specimen was used for all tests. a The S-gene amplicon (S) was compared with ORF E (E) in singleplex and duoplex reactions. b The direct performance comparison of ORF E (E) amplicons with ORF N (N) and RdRP (R) demonstrated the differences in sensitivity, whereby E>N>R

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