Fig. 4
From: Nup133 and ERα mediate the differential effects of hyperoxia-induced damage in male and female OPCs
Nup133 directly interacts with ERα. a Western blot analysis of male and female OPCs with anti-ERα and anti-ERβ antibodies at normal (3% O2) conditions and 24 h post 80%O2 shock, showing a significant increase in ERα expression in the female OPCs. b Western blot results of co-immunoprecipitation performed using anti-Nup133 antibody showing the interaction between ERα and Nup133 in male and female OPCs under normoxia and hyperoxia. c Western blot results showing Nup133 expression post treatment of male and female OPCs with 4,4′,4″-(4-Propyl-[1H]-pyrazole-1,3,5-triyl) trisphenol (PPT) and ICI 182,780 (ICI) under normoxia and 24 h hyperoxia. d RT-qPCR results showing Nup133 mRNA expression changes post 17-β estradiol (E2) treatment in male and female OPCs under normoxia and hyperoxia. e RT-qPCR results showing Nrf1 mRNA expression changes post 17-β estradiol (E2) treatment in male and female OPCs under normoxia and hyperoxia. f Functional categorization of significantly enriched proteins in male and female OPCs post 24 h 80% O2 and E2 treatment using IPA. Bar graphs depict the most extensively enriched biological processes among the altered proteins. Cutoff p value < 0.05 (Fisher’s exact test). “C” represents control. Data is representative of five independent experiments. All other data are representative of three independent experiments. Bars and error represent mean ± SEM of replicate measurements. *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001 (Student’s t test)