Fig. 3

Identification of Nup133 targets in male and female OPCs. a Line plot representation of ChIP-Seq signal density for Nup133 male and female ChIP and input controls centered on predicted TSS. b Pie chart showing proportions of targets with protein-coding or non-protein-coding designation. c Integrative Genomics Viewer (IGV) visualization of Nup133 occupancy at selected target sites related to oligodendrocyte differentiation. ChIP-Seq data are representative of four independent experiments. d Known motif analysis of Nup133-binding regions identified by the software HOMER. The highest significant motif was identified as Nrf1 and found to be common in both male and female groups. q value determine by Benjamini correction was 0.0000 for all the represented motifs. e mRNA expression validation of Nrf1 showing downregulation in male OPCs and a slight upregulation in female OPCs post 24 h hyperoxia. Real-time PCR data is representative of three independent experiments. f Western blot of Nup133 and Nrf1 showing +1 for Nup133 specific siRNA 1, +2 for Nup133 specific siRNA 2,—for scrambled siRNA and Ctrl for non-transfected cell lysate. Transfection was performed in the OLN93 cells with 48 h siRNA incubation. Data is representative of three independent experiments. g RT-qPCR analysis of Nup133 expression following siRNA transfection for 48 h. Scrambled siRNA (siRNA NC) and untreated sample (Ctrl) are used as controls. Data is representative of three independent experiments