Fig. 2
From: Nup133 and ERα mediate the differential effects of hyperoxia-induced damage in male and female OPCs
Nuclear envelope (NE) proteins are downregulated in male OPCs as a result of hyperoxia. a STRING protein interaction analysis of the significantly altered proteins (p < 0.05) in the male (hyperoxia vs normoxia) and female (hyperoxia vs normoxia) groups, with an interaction score of highest confidence (0.900), showing the nuclear envelope proteins to be altered only in the male group. b Western blot analysis of male and female OPCs with anti-Nup133 antibody under normal (3% O2) conditions and post 24 h 80% O2 treatment, showing a significant decrease in expression in the male OPCs. Whereas in female OPCs, Nup133 protein expression showed a trend towards upregulation. ***p < 0.001, **p < 0.01, *p < 0.05 (Student’s t test), n = 3. Values are means ± SEM. c mRNA expression of Nup133 showing downregulation in male OPCs and upregulation in female OPCs post-hyperoxia. ***p < 0.001, **p < 0.01, *p < 0.05 (Student’s t test), n = 3. Values are means ± SEM. d Representative images of mouse male and female OPCs stained for Nup133 after treatment under normal oxygen conditions and post 24 h 80% O2 shock. Scale bar represents 25 μm