Table 1 Summary of all observed marker on granulocyte and monocytes and their subpopulations

Granulocytes

CD11b+ (%)

98.94 (97.83–99.78)

95.27 (29.87–97.73)

0.0001

CD11b+ (MFI)

3423 (2274–6123)

2936 (1847–5151)

0.5974

CD16+ (%)

98.05 (93.91–99.32)

89.59 (28.21–96.12)

0.0002

CD16+ (MFI)

58.687 (40.735–76.909)

26.939 (26.309–36.276)

0.0001

CD32+ (%)

99.53 (98.11–99.85)

97.14 (32.18–99.41)

0.0017

CD32+ (MFI)

16.466 (12.504–27.052)

19.779 (11.886–24.551)

0.3418

CD62L+ (%)

96.55 (93.15–99.34)

85.07 (32.05–92.36)

0.0001

CD62L+ (MFI)

43.841 (21.105–64.313)

19.172 (12.638–31.370)

0.0028

TLR2+ (%)

2.9 (0.6–10.98)

8.36 (3.32–72.73)

0.0035

TLR2+ (MFI)

1506 (190.9–5427)

778.7 (365.3–3335)

0.8053

Granulocytes subsets

CD16+ CD62L+

93.01 (87.46–96.55)

74.37 (25.78–81.35)

0.0001

CD16+ CD62L−

3.24 (0.54–8.1)

6.68 (0.21–16.0)

0.2453

CD16dim CD62L+

0.425 (0.25–1.18)

1.06 (0.37–3.8)

0.0092

CD16+CD62L+ (classical)

CD11b+ (%)

100.0 (98.67–100)

99.40 (97.76–100)

0.0545

CD11b+ (MFI)

3439 (2284–6139)

2978 (1880–5259)

0.5974

CD32+ (%)

100.0 (100.0–100.0)

100.0 (99.97–100.0)

-

CD32+ (MFI)

16.655 (12.794–27.373)

20.943 (13.267–27.917)

0.098

TLR2+ (%)

0.985 (0.32–11.52)

5.75 (0.46–79.95)

0.0346

TLR2+ (MFI)

1598 (360.3–5217)

1013 (613.4–3321)

0.8603

CD16+CD62L- (anti-inflammatory)

CD11b+ (%)

98.43 (94.62–99.85)

98.17 (92.92–99.48)

0.3787

CD11b+ (MFI)

3332 (2040–5834)

2753 (1686–3902)

0.3418

CD32+ (%)

99.99 (99.61–100.0)

99.96 (54.96–100.0)

0.0587

CD32+ (MFI)

15.244 (11.030–26.700)

19.774 (13.674–27.184)

0.0726

TLR2+ (%)

0.98 (0.27–6.67)

5.97 (0.57–81.36)

0.0183

TLR2+ (MFI)

2028 (443.6–7026)

941.5 (634.4–3216)

0.4181

CD16dimCD62L+ (pro-inflammatory)

CD11b+ (%)

98.46 (81.77–100)

97.05 (82.95–99.86)

0.5495

CD11b+ (MFI)

2457 (1516–5741)

2731 (2171–4653)

0.3787

CD32+ (%)

100 (98.5–100.0)

99.97 (99.36–100.0)

0.9697

CD32+ (MFI)

8236 (5792–16.004)

7168 (4230–10.209)

0.5035

TLR2+ (%)

1.4 (0.0–5.5)

9.18 (0.73–35.08)

0.0043

TLR2+ (MFI)

2625 (0.00–5333)

657.8 (446.7–3439)

0.0378

Monocytes

CD11b+ (%)

99.51 (98.50–99.96)

98.64 (95.76–99.33)

0.0137

CD11b+ (MFI)

2694 (1256–6598)

2093 (993.4–4453)

0.1489

CD16+ (%)

9.33 (5.91–11.38)

21.26 (10.97–49.66)

0.0003

CD16+ (MFI)

12.126 (5745–19.915)

3684 (1817–5825)

0.0002

CD32+ (%)

99.93 (99.04–99.98)

99.65 (92.43–99.82)

0.0724

CD32+ (MFI)

22.733 (12.835–31.849)

19.335 (13.609–30.177)

0.5035

CD62L (%)

94.24 (55.77–96.31)

80.15 (27.67–96.15)

0.0083

CD62L+ (MFI)

28.574 (15.121–61.757)

11.974 (5130–25.963)

0.0011

HLA-DR+ (%)

99.51 (97.14–99.83)

94.46 (85.72–97.56)

0.0002

HLA-DR+ (MFI)

5050 (3839–5678)

2008 (1135–4030)

0.0002

TLR2+ (%)

99.07 (95.67–99.83)

97.27 (92.02–99.30)

0.0317

TLR2+ (MFI)

3693 (2511–5553)

3166 (1271–5754)

0.1131

CD14+ CD16+

5.925 (4.47–9.33)

17.52 (9.89–45.38)

0.0001

CD14+CD16-

90.59 (88.62–94.09)

79.4 (50.34–88.55)

0.0001

CD14dim CD16+

2.1 (1.12–5.77)

1.74 (0.35–5.34)

0.1299

CD14+CD16- (classical)

CD11b+ (%)

99.77 (98.59–99.98)

98.26 (94.89–99.29)

0.0006

CD11b+ (MFI)

2709 (1250–6706)

2025 (914.2–4189)

0.13

CD32+ (%)

99.94 (99.27–99.99)

99.67 (93.55–99.8)

0.0183

CD32+ (MFI)

22.650 (12.310–31.390)

18.040 (12.880–27.480)

0.2453

CD62L+ (%)

96.81 (57.66–98.74)

82.53 (26.2–98.1)

0.0054

CD62L+ (MFI)

29.690 (15.240–64.070)

12.150 (5195–28.160)

0.0014

HLA-DR+ (%)

99.57 (98.29–99.87)

95.59 (88.84–99.24)

0.0003

HLA-DR+ (MFI)

4260 (3050–4736)

1833 (907.4–2658)

0.0001

TLR2+ (%)

99.69 (97.23–99.96)

97.89 (94.51–99.29)

0.0151

TLR2+ (MFI)

3671 (2465–5485)

2944 (1225–5206)

0.0845

CD14+CD16+ (anti-inflammatory)

CD11b+ (%)

99.85 (97.72–100.0)

99.92 (98.06–100.0)

0.5714

CD11b+ (MFI)

3009 (1441–6496)

2643 (1357–5016)

0.4181

CD32+ (%)

99.93 (96.42–100.0)

99.77 (90.23–99.94)

0.4165

CD32+ (MFI)

24.030 (14.400–40.560)

22.930 (16.130–34.550)

0.6985

CD62L+ (%)

74.74 (35.97–86.96)

75.91 (42.13–95.42)

0.5973

CD62L+ (MFI)

16.240 (11.660–23.330)

12.150 (4641–20.090)

0.0221

HLA-DR+ (%)

99.16 (87.21–100.0)

93.53 (64.45–96.81)

0.0378

HLA-DR+ (MFI)

14.880 (10.390–19.610)

3219 (2243–7424)

0.0001

TLR2+ (%)

98.54 (82.51–99.77)

95.53 (87.48–99.86)

0.5495

TLR2+ (MFI)

4893 (3280–6613)

3546 (1492–6474)

0.0528

CD14^dimCD16+ (pro-inflammatory)

CD11b+ (%)

98.17 (89.87–99.83)

96.82 (86.89–98.53)

0.3418

CD11b+ (MFI)

1523 (803.7–3416)

1250 (1027–2207)

0.5974

CD32+ (%)

98.87 (85.65–100.0)

96.11 (83.81–100.0)

0.09

CD32+ (MFI)

15.860 (10.070–34.790)

14.650 (11.360–22.380)

0.2178

CD62L+ (%)

32.15 (13.08–44.67)

34.89 (5.3–53.33)

0.8053

CD62L+ (MFI)

9512 (4503–31.870)

16.650 (7670–25.480)

0.1131

HLA-DR+ (%)

92.48 (75.53–98.71)

63.51 (46.67–95.24)

0.0151

HLA-DR+ (MFI)

12.550 (7028–15.170)

8144 (3757–11.960)

0.0017

TLR2+ (%)

84.39 (61.21–97.33)

76.67 (41.11–91.01)

0.1697

TLR2+ (MFI)

3529 (2709–5068)

3104 (1390–4592)

0.13