Fig. 2
From: Gene correction of HBB mutations in CD34+ hematopoietic stem cells using Cas9 mRNA and ssODN donors
Gene correction of HBBIVS1–110 in CD34+ HSCs using CRISPR/Cas9 and ssODNs. a CD34+ HSCs co-nucleofected with pX330.sg HBBIVS1–110 plasmid and 5TS ssODN. Indel frequency was measured by TIDE analysis and 3% of 6 bp insertions were spotted. b Single-colony sequencing analysis samples showed successful gene insertion of NheI restriction-site tag to the HBB gene by HDR in pX330.sg HBBIVS1–110/5TS ssODN-treated sample. c Indel and HDR rate of CD34+ HSCs co-nucleofected with specified ssODN and Cas9 mRNA measured by T7 assay and RFLP assay in Bioanalyzer using DNA1000 kit. d TIDE analysis exhibited the significant enrichment of 6 bp insertions up to 8%. e The absolute quantification by next-generation analysis displayed the significant enrichment of 6 bp insertions up to 8% (N = 1)