Fig. 1
From: Gene correction of HBB mutations in CD34+ hematopoietic stem cells using Cas9 mRNA and ssODN donors
Comparison of three different gene-editing tools at HBB promoter and targeting HBBIVS1–110 locus. a HEK-293 cells were transfected with 500, 1000, and 1500 ng of expression plasmid of either ZFNs, TALENs, or CRISPR/Cas9 targeting the promoter of the β-globin gene locus. The indel rate was measured by T7 endonuclease-I (T7EI) assay. Results represent mean values for each concentration, and significant difference was observed among the tools used (P < 0.0001). b Design of HBBIVS1–110 sgRNA and ssODN donor templates. K562 cells electroporated with pX330.sg HBBIVS1–110 plasmid measured for indel rate and HDR. The experiment results from T7EI assay and RFLP assay (c, d) plotted as a bar graph against utilized ssODNs. c The results of T7EI assay analyzed by 1.5% agarose gel electrophoresis. d The results of RFLP assay measured in Bioanalyzer using DNA1000 kit (N = 3)