Fig. 3

Cytokine induced CIS mRNA expression and STAT5 phosphorylation according to CISH haplotypes. a CIS mRNA expression of PBMCs from healthy adult donors with distinct haplotypes (circles: homozygous H1 carriers (H1/H1), triangles: H2 carriers (H1/H2, H2/H2), squares: H3 carriers (H1/H3)) treated with IL-2 (left graph) or IL-7 (right graph) for 1 and 2 h. CIS mRNA levels—calculated in comparison to the housekeeping gene GAPDH—are normalized against time point 0 for each donor. Median and IQR of 2^−ΔΔCT values are indicated. p value for effect of IL-2/IL-7 stimulation on CIS mRNA levels considering all genotypes (Friedman test) is indicated as *** for p < 0.001. For IL-2 stimulation, 5 H1/H1, 5 H2, and 3 H3 carriers were included. For IL-7 stimulation, 6 H1/H1, 5 H2, and 1 H3 carriers were analyzed. b IL-2 and IL-7 induced STAT5 phosphorylation of CD4⁺ T cells for different CISH haplotypes. Representative gating procedure on CD4⁺ T cells for pSTAT5 expression without stimulation (left chart), after stimulation with IL-2 (middle chart) and IL-7 (right chart) is shown in the upper plots. Mean fluorescence intensity values (MFI) for pSTAT5 are indicated. In the bottom charts, ∆ pSTAT5 values (MFI) (difference to pSTAT5 level in absence of IL-2 or IL-7) after stimulation with IL-2 (left panel) and IL-7 (right panel) are depicted for the different haplotype carriers. Medians are indicated. No statistically significant differences were detected between study groups (Mann-Whitney U test, two-tailed)