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Fig. 1 | Molecular and Cellular Pediatrics

Fig. 1

From: Role of second-hand smoke (SHS)-induced proteostasis/autophagy impairment in pediatric lung diseases

Fig. 1

SHS exposure induces autophagy-impairment in pediatric and neonate murine lungs mice lungs. a, c Western blots showing the accumulation of ubiquitinated proteins (ubiquitin, Ub), p62 (sequestosome-1), VCP (valosin containing protein), and HIF-1α (hypoxia inducible factor) in the soluble and/or insoluble lung protein-fractions isolated from SHS (CS) or room air (RA)—exposed neonatal mice sacrificed at day 1 (P1) or 14 (P14), post-delivery. The mothers of these pups were also exposed to SHS for the last 14 days of the pregnancy. b Bar graph of relative optical density of soluble Ub, VCP, p62, and HIF-1α normalized to β−actin. d Bar graph of relative optical density of insoluble Ub, VCP, and p62 is also normalized to soluble β-actin. (*p < 0.05; **p < 0.01)

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