Table 1 Primer sequences of FOXP3 and CXCL12 genes
From: Wilms’ tumor susceptibility: possible involvement of FOXP3 and CXCL12 genes
Primer sequence | PCR product | ||
|---|---|---|---|
RFLP-PCR | rs1801157 | 5′-CAGTCAACCTGGGCAAAGCC-3′ | 293 bp |
CXCL12 | 5′-CCTGAGAGTCCTTTTGCGGG-3′ | ||
AS-PCR | 5′-CTGGCTCTCTCCCCAACTGA-3′ | Allele A 334 bp | |
rs3761548 | 5′-ACAGAGCCCATCATCAGACTCTCTA-3′ | ||
FOXP3 | 5′-TGGCTCTCTCCCCAACTGC-3′ | Allele C 333 bp | |
5′-ACAGAGCCCATCATCAGACTCTCTA-3′ | |||
5′-CCCAGCTCAAGAGACCCCA-3′ | Allele A 442 bp | ||
rs2232365 | 5′-GGGCTAGTGAGGAGGCTATTGTAAC-3′ | ||
FOXP3 | 5′-CCAGCTCAAGAGACCCCG-3′ | Allele G 427 bp | |
5′-GCTATTGTAACAGTCCTGGCAAGTG-3′ | |||
Sequencing | rs3761548 | 5′-TCTCCGTGCTCAGTGTAGAA-3′ | 330 bp |
FOXP3 | 5′-AACTAGGCCTCCTGACCTATG-3′ | ||
rs2232365 | 5′-AGAAGGAGTGGGCATTTGAG-3′ | 284 bp | |
FOXP3 | 5′-GCAGGTGTAGATAGACATGAAGAG-3′ |