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Fig. 1 | Molecular and Cellular Pediatrics

Fig. 1

From: Milk glucosidase activity enables suckled pup starch digestion

Fig. 1

Dahlqvist disaccharidase assays from the mid-jejunum of suckling and weaned mice with Mgam KO or WT genotypes (n = 8). Weaning was by separation from the litter at 21 days. Tissues were collected at 13 days (suckling) and 90 days (weaned). The lumen was first flushed with EDTA PBS buffer, and then, tissue was collected and homogenized in PBS buffer and analyzed as unit per milligram protein (a). The luminal flush (b) was assayed for activity per milliliter of collected fluid. Note that the homogenate activities for maltose, sucrose, and isomaltose substrates increased after weaning in the WT but not in the null mice. Note also the difference in ordinate scales between (a) and (b)

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