Figure 5

Relative expression of ATF6 in CF versus non- CF bronchial epithelial cell lines and bronchial brushings. Relative expression of ATF6 mRNA was determined by qRT-PCR and normalised to β-actin in the CF bronchial epithelial cell line CFBE41o- compared with its non-CF counterpart 16HBE14o- (three separate cultures tested in triplicate) and in bronchial brushings (CF, n = 8; non-CF, n = 9). Data are represented as mean ± SEM and were compared by t test (*p < 0.05, **p < 0.01). All qRT-PCR experiments for cell lines were performed in triplicate and included no-template controls.