Figure 4
From: miRNA-221 is elevated in cystic fibrosis airway epithelial cells and regulates expression of ATF6
miR-145, miR-221 and miR-494 target human ATF6. Relative luciferase activity in HEK293 cells (1 × 105 in triplicate) transiently co-transfected with full-length 408 bp human ATF6 3′UTR firefly luciferase reporter plasmid, a constitutive Renilla luciferase reporter plasmid (pRLSV40) and scrambled control (Scr), premiR (PM)-223 control, PM-145, PM-221 and PM-494. Firefly luciferase activity was normalised to Renilla luciferase activity. Transfection efficiency was monitored by visualisation of endogenous RFP expressed from the ATF6 3′UTR reporter plasmid where uniform transfection (estimated to be approximately 50%) was observed across all wells. Data are represented as mean ± SEM and were compared by t test (*p < 0.05). Data is representative of three experiments.