Relative expression levels of miR-221 in CF versus non-CF bronchial epithelial cell lines and bronchial brushings. (A) Relative expression of miR-221 was determined by qRT-PCR using individual TaqMan human miRNA assays and normalised to miR-218 in the CF bronchial epithelial cell line CFBE41o- compared with its non-CF counterpart 16HBE14o- (three separate cultures measured in triplicate) and in bronchial brushings (CF, n = 8; non-CF, n = 9). Data are represented as mean ± SEM and were compared by t test (*p < 0.05, **p < 0.01). All qRT-PCR experiments for cell lines were performed in triplicate and included no-template controls. (B) Relative expression of miR-145 and miR-494 normalised to β-actin in the CF versus non-CF cell lines and bronchial brushings used in (A).