Fig. 5

Pyrin is involved in mediating cell death. Neutrophils (5 × 10⁶/ml) from HC (healthy control) and FMF patients were stained with SytoxGreen and incubated for 4 h without or with TCDA (0.5 mg/ml). Supernatants were harvested and measured for S100A12 protein content by ELISA (a) and for LDH using the LDH assay kit according to the manufacturer’s protocol (b). Data are mean and SD of 6–8 independent individuals (b). All FMF biosamples were from homozygous patients. Statistical significance was calculated Brown-Forsythe and Welch’s ANOVA test followed by Dunn’s multi-comparison test (* = p > 0.05, ** = 0.005, *** = p > 0.0005)