Fig. 3

FMF neutrophils spontaneously undergo cell death and release S100A12. Neutrophils from HC (healthy control) and FMF patients were isolated as described and either seeded at 1 × 10⁶/ml or 5 × 10⁶/ml in microtiter plates after staining with SytoxGreen (2.5 μM). Cell death was quantified as amount of fluorescence at 523 nm over time (up to 4 h) (a). b Supernatants of 4-h culture were measured for S100A12 content using ELISA. c Supernatants of a (4-h culture) were measured for LDH content to independently quantify cell death. All FMF biosamples were from homozygous patients. All experiments were done at least 4 times (n = 4–7) and statistical significance was calculated Brown-Forsythe and Welch’s ANOVA test followed by Dunn’s multi-comparison test (* = p > 0.05, ** = 0.005, *** = p > 0.0005)