Fig. 2
Imaging cell death in FMF neutrophils. Neutrophils from peripheral blood were isolated as described and were stained with 2.5 μM SytoxGreen. 1 × 106/ml neutrophils were either left untreated or were stimulated (a) with PMA (b, 100 nM) or MSU crystals (c, 200 μg/ml) for 4 h. Then, photograph of cells was taken from image of fluorescence microscopy at 488 nm, scale bar 50 μm is shown (a–c). In the next experiment, 5 × 106/ml neutrophils were either stimulated with PMA or left untreated for 4 h. Then cells were analyzed by fluorescence microscopy and photograph was taken at wavelength of 488 nm, magnification, scale bar of 100 μm is shown (d–g). Representative images are shown and scale bars are indicating cell size. All FMF biosamples were from homozygous patients