Mechanism of human rhinovirus infections
© The Author(s). 2016
Received: 20 January 2016
Accepted: 24 May 2016
Published: 1 June 2016
About 150 human rhinovirus serotypes are responsible for more than 50 % of recurrent upper respiratory infections. Despite having similar 3D structures, some bind members of the low-density lipoprotein receptor family, some ICAM-1, and some use CDHR3 for host cell infection. This is also reflected in the pathways exploited for cellular entry. We found that even rhinovirus serotypes binding the same receptor can travel along different endocytic pathways and release their RNA genome into the cytosol at different locations. How this may account for distinct immune responses elicited by various rhinoviruses and the observed symptoms of the common cold is briefly discussed.
KeywordsNasal epithelium Human rhinoviruses Rhinovirus receptors Immune response
Human rhinoviruses (HRVs) account for more than 50 % of upper respiratory tract infections. The disease is known as the common cold that usually resolves within 5–7 days. Symptoms include nasal stuffiness, sneezing, coughing, and a sore throat but about 12–32 % of HRV infections in children of less than 4 years are asymptomatic . Treatment is so far only palliative as no vaccination and approved antivirals are available; because of the usually annoying but uncomplicated course of the disease, only drugs without side effects will be accepted by otherwise healthy patients. However, rhinovirus infections on top of chronic obstructive pulmonary disease (COPD), asthma, or cystic fibrosis (CF) might become life-threatening increasing the demand for the development of such antivirals .
Pre-school children can experience an upper respiratory infection up to 8 to 12 times per year (reviewed in ) that might lead to wheezing, otitis media, bronchiolitis, exacerbations of asthma, CF, or COPD and aggravate allergic reactions. The newly discovered RV-C species is thought to account for a significant proportion of HRV-related illness, especially in infants .
The nasal epithelium
The main site of RV infections is the nasal mucosa. The nasal cavity is lined by a pseudostratified epithelium composed of columnar, ciliated epithelial cells, mucous-secreting goblet cells, and basal cells ; lymphocytes, mast cells, dendritic cells, and macrophages migrate to and then home in the epithelium under pathologic conditions. The epithelium is anchored in the underlying extracellular matrix that contains vascular endothelial cells and submucosal glands. The luminal, ciliary surface of the airways is covered by periciliary liquid and a mucus layer trapping inhaled particles such as bacteria and viruses. Mucus produced by the glands and goblet cells contains water, ions (e.g., Na+, Cl−, and K+), glycoproteins, and immunoglobulins such as IgG and polymeric IgA (pIgA) . Beating cilia transport the mucus layer together with adhering particles to the oral cavity where it is swallowed; digestion then leads to destruction of the infectious agent. Mucociliary clearance requires a balance between ciliary beat, volume, and composition of mucus and periciliary fluid. This balance is perturbed in chronic inflammatory lung diseases such as CF and COPD. In CF, mucus composition, viscosity, and pH (a mean of 6.57 versus 7.18 in controls) are altered, rendering the airways more susceptible to infections .
HRV receptors, entry, and replication
HRVs are non-enveloped with a ss(+)RNA genome that is protected by an icosahedral protein capsid built of 60 copies each of the four viral proteins VP1–VP4 . Based on phylogeny, more than 150 HRV types are classified as species A, B, and C . Twelve HRV-A (the minor group) bind members of the low-density lipoprotein receptor (LDLR) family whereas the remaining A and B types (the major group) bind intercellular adhesion molecule-1 (ICAM-1) [9, 10]; for HRV-C, the recently identified CDHR3 might serve as a receptor . The mechanisms of entry and uncoating of HRV-C are unknown; we will thus limit the discussion to HRV-A and B.
For infection, the cognate receptor must be accessible to the virus, i.e., at the apical surface of ciliated epithelial cells. While reports on the location of ICAM-1 in the healthy nasal mucosa are contradictory, it is generally agreed that this receptor is upregulated upon inflammation . Re-investigating this issue, we detected ICAM-1 at the ciliated surface of all nasal epithelial cells in the nasal tissue from healthy individuals (Ellinger et al., to be published). As expected from its “normal” physiologic function, LDLR is located at the basolateral plasma membrane of the polarized airway, intestinal, renal, and hepatic cell lines. We were thus surprised to find that LDLR and LDLR-related protein 1 (LRP-1) are present at the apical side of the nasal epithelial cells and thus available for uptake of virus at its main port of entry (Ellinger et al., to be published).
Once the RNA has arrived in the cytoplasm, it is translated into a polyprotein. After autocatalytic cleavage into the structural (capsid) and non-structural proteins, the RNA is replicated by the viral polymerase. Finally, infectious progeny is assembled and released into the nasal cavity . In contrast to HRV infection in tissue culture cells, airway epithelial cells of patients are not lysed for virus release; as shown for other enteroviruses, it is thus possible that cell-to-cell spread might occur via virus-carrying microvesicles .
Host response to HRV infections
Although initially believed that HRV infection was limited to the upper airways, replicating virus was found in ciliated epithelial cells of the lower respiratory tract. Infected cells appear in patches, and only 10 % of the ciliated cells produce viral proteins and RNA . Similar results had been obtained with in situ hybridization in nasal biopsies, again indicating that only a small proportion of cells were infected . Nevertheless, basal cells are more susceptible to infection as compared to fully differentiated ciliated cells . This might be related to the higher expression level of ICAM-1 in basal cells versus ciliated cells (Fuchs, unpublished observations). The absence of visible cytopathic alterations in the airway epithelium led to the hypothesis that the symptoms are rather due to the immune response of the host . Upon HRV entry into and replication in ciliated epithelial cells, signalling pathways are activated leading to the release of various cytokines (IL-1ß, TNF, IL-8, IL-6, IL-11), chemokines (Rantes, MCP-1, MP-10), vasoactive peptides (bradykinin), and growth factors (VFGF) . Consequently, inflammatory cells (leukocytes, granulocytes, monocytes) become activated and invade the submucosa. This results in amplification of the inflammatory process and the typical symptoms of the common cold. Conversely, HRV infections are controlled by innate and adaptive immune responses. Type-I interferons are the early mediators of the innate immune system, while neutralizing IgA and IgG in serum and secretions are observed 1–2 weeks after infection as a consequence of the adaptive immune response. Nasal epithelial cells express the pIgA receptor (pIgR) and the neonatal Fc-receptor (FcRn) that transport the respective immunoglobulins into nasal secretions . Expression of FcRn in the ciliated epithelial cells and in dendritic cells (DC) in the nasal mucosa might contribute to mucosal immunity as shown for FcRn in the intestine .
How could the entry pathway taken by a given rhinovirus impact on the immune response? We presented evidence for HRV-A2 transferring its genome into the cytosol via a pore in the membrane and the remaining empty capsid being directed towards lysosomes where it is degraded. On the other hand, HRV-B14 breaks the endosomal membrane resulting in arrival of viral proteins in the cytoplasm . As a consequence, one might hypothesize that the proteins of incoming virus are presented to the immune system either as products of proteasomal (HRV-B14) or lysosomal (HRV-A2) processing. Degradation products of the former would thus be mainly presented by the MHC-I system and the latter mainly by the MHC-II system. Furthermore, pattern recognition receptors in the endosome are different from those in the cytosol . Such differences might impact on differences in the primary immune response; nevertheless, in the absence of further experimentation, this remains pure speculation.
Summary and conclusions
HRVs are a major cause of respiratory infections of infants. The numerous serotypes are precluding the development of a vaccine, and current treatments only palliate the symptoms. HRVs enter from the apical side of the cells lining the airways by receptor-mediated endocytosis via LDLR and ICAM-1. We demonstrated that all nasal epithelial cells express ICAM-1 and LDLR at their ciliated side and that two ICAM-1 binding HRV types exhibit different temperature dependence of uncoating and take distinct routes inside the cell for genome release (Fig. 1). This may explain why different HRVs elicit different signals during cell entry resulting in different host responses to infection [24, 25]. Understanding the details of receptor binding, entry, and uncoating is crucial for identifying novel means of fighting the common cold.
This study is funded by the Austrian Science Fund P-274444-B13.
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- Jacobs SE, Lamson DM, St George K, Walsh TJ (2013) Human rhinoviruses. Clin Microbiol Rev 26:135–162View ArticlePubMedPubMed CentralGoogle Scholar
- Thibaut HJ, Lacroix C, De Palma AM, Franco D, Decramer M, Neyts J (2016) Toward antiviral therapy/prophylaxis for rhinovirus-induced exacerbations of chronic obstructive pulmonary disease: challenges, opportunities, and strategies. Rev Med Virol 26:21–33View ArticleGoogle Scholar
- Turner RB (1997) Epidemiology, pathogenesis, and treatment of the common cold. Ann Allergy Asthma Immunol 78:531–539View ArticlePubMedGoogle Scholar
- Miller EK (2010) New human rhinovirus species and their significance in asthma exacerbation and airway remodeling. Immunol Allergy Clin North Am 30:541–552View ArticlePubMedPubMed CentralGoogle Scholar
- Knight DA, Holgate ST (2003) The airway epithelium: structural and functional properties in health and disease. Respirology 8:432–446View ArticlePubMedGoogle Scholar
- Yoshida M, Kobayashi K, Kuo TT, Bry L, Glickman JN, Claypool SM, Kaser A, Nagaishi T, Higgins DE, Mizoguchi E, Wakatsuki Y, Roopenian DC, Mizoguchi A, Lencer WI, Blumberg RS (2006) Neonatal Fc receptor for IgG regulates mucosal immune responses to luminal bacteria. J Clin Invest 116:2142–2151View ArticlePubMedPubMed CentralGoogle Scholar
- Song Y, Salinas D, Nielson DW, Verkman AS (2006) Hyperacidity of secreted fluid from submucosal glands in early cystic fibrosis. Am J Physiol Cell Physiol 290:C741–C749View ArticlePubMedGoogle Scholar
- Palmenberg AC, Spiro D, Kuzmickas R, Wang S, Djikeng A, Rathe JA, Fraser-Liggett CM, Liggett SB (2009) Sequencing and analyses of all known human rhinovirus genomes reveal structure and evolution. Science 324:55–59View ArticlePubMedPubMed CentralGoogle Scholar
- Staunton DE, Merluzzi VJ, Rothlein R, Barton R, Marlin SD, Springer TA (1989) A cell adhesion molecule, ICAM-1, is the major surface receptor for rhinoviruses. Cell 56:849–853View ArticlePubMedGoogle Scholar
- Hofer F, Gruenberger M, Kowalski H, Machat H, Huettinger M, Kuechler E, Blaas D (1994) Members of the low densitylipoprotein receptor family mediate cell entry of a minor-group common cold virus. Proc Nat Acad Sci USA 91:1839–1842View ArticlePubMedPubMed CentralGoogle Scholar
- Bochkov YA, Watters K, Ashraf S, Griggs TF, Devries MK, Jackson DJ, Palmenberg AC, Gern JE (2015) Cadherin-related family member 3, a childhood asthma susceptibility gene product, mediates rhinovirus C binding and replication. Proc Natl Acad Sci U S A 112:5485–5490View ArticlePubMedPubMed CentralGoogle Scholar
- Winther B, Arruda E, Witek TJ, Marlin SD, Tsianco MM, Innes DJ, Hayden FG (2002) Expression of ICAM-1 in nasal epithelium and levels of soluble ICAM-1 in nasal lavage fluid during human experimental rhinovirus infection. Arch Otolaryngol Head Neck Surg 128:131–136View ArticlePubMedGoogle Scholar
- Fuchs R, Blaas D (2010) Uncoating of human rhinoviruses. Rev Med Virol 210:281–297View ArticleGoogle Scholar
- Jurgeit A, Moese S, Roulin P, Dorsch A, Lotzerich M, Lee WM, Greber UF (2010) An RNA replication-center assay for high content image-based quantifications of human rhinovirus and coxsackievirus infections. Virol J 7:264View ArticlePubMedPubMed CentralGoogle Scholar
- Casasnovas JM, Springer TA (1994) Pathway of rhinovirus disruption by soluble intercellular adhesion molecule 1 (ICAM-1): an intermediate in which ICAM-1 is bound and RNA is released. J Virol 68:5882–5889PubMedPubMed CentralGoogle Scholar
- Nurani G, Lindqvist B, Casasnovas JM (2003) Receptor priming of major group human rhinoviruses for uncoating and entry at mild low-pH environments. J Virol 77:11985–11991View ArticlePubMedPubMed CentralGoogle Scholar
- Inal JM, Jorfi S (2013) Coxsackievirus B transmission and possible new roles for extracellular vesicles. Biochem Soc Trans 41:299–302View ArticlePubMedGoogle Scholar
- Mosser AG, Brockman-Schneider R, Amineva S, Burchell L, Sedgwick JB, Busse WW, Gern JE (2002) Similar frequency of rhinovirus-infectible cells in upper and lower airway epithelium. J Infect Dis 185:734–743View ArticlePubMedGoogle Scholar
- Arruda E, Boyle TR, Winther B, Pevear DC, Gwaltney JM Jr, Hayden FG (1995) Localization of human rhinovirus replication in the upper respiratory tract by in situ hybridization. J Infect Dis 171:1329–1333View ArticlePubMedGoogle Scholar
- Jakiela B, Brockman-Schneider R, Amineva S, Lee WM, Gern JE (2008) Basal cells of differentiated bronchial epithelium are more susceptible to rhinovirus infection. Am J Respir Cell Mol Biol 38:517–523View ArticlePubMedPubMed CentralGoogle Scholar
- Kennedy JL, Turner RB, Braciale T, Heymann PW, Borish L (2012) Pathogenesis of rhinovirus infection. Curr Opin Virol 2:287–293View ArticlePubMedPubMed CentralGoogle Scholar
- Heidl S, Ellinger I, Niederberger V, Waltl EE, Fuchs R (2015) Localization of the human neonatal Fc receptor (FcRn) in human nasal epithelium. Protoplasma. doi:10.1007/s00709-015-0918-y PubMedGoogle Scholar
- Rath T, Baker K, Pyzik M, Blumberg RS (2014) Regulation of immune responses by the neonatal fc receptor and its therapeutic implications. Front Immunol 5:664View ArticlePubMedPubMed CentralGoogle Scholar
- Wark PA, Grissell T, Davies B, See H, Gibson PG (2009) Diversity in the bronchial epithelial cell response to infection with different rhinovirus strains. Respirology 14:180–186View ArticlePubMedGoogle Scholar
- Schuler BA, Schreiber MT, Li L, Mokry M, Kingdon ML, Raugi DN, Smith C, Hameister C, Racaniello VR, Hall DJ (2014) Major and minor group rhinoviruses elicit differential signaling and cytokine responses as a function of receptor-mediated signal transduction. PLoS One 9:e93897View ArticlePubMedPubMed CentralGoogle Scholar